Species mapping Since health estimates developed from hyperspectral data have been species specific in our particular projects, we must first determine the species composition of each image pixel. Two approaches are taken to develop species maps - depending on the spatial resolution. Both methods rely on the careful development of an endmember library in which the endmember spectra represent the species of interest. In our work, we develop these spectra from the imagery itself, by careful geolocation of each tree canopy and extraction of those spectra representing healthy examples of each species. Spectral Unmixing For coarse resolution imagery (10m+), each pixel may contain a number of tree canopies, and the species may vary within the pixel. In this situation, we use mixture tuned matched filtering to determine the abundance of each species within each pixel. Just a note to us re the way mtmf works: Mapping the pixel-by-pixel relative abundance of vegetation was carried out using mixture-tuned matched-Wltering (MTMF) (Kruse et al. 2003). MTMF expands on a proven signal processing methodology (matched-Wltering; MF) that maximizes the response to known endmembers while suppressing the response of the composite background. MTMF adds to MF an infeasibility score (IS) that measures the likelihood that a specific pixel is a feasible mixture of the target endmember and the background distribution defned by the full scene covariance. The MF output from MTMF ranges from 0.0 to 1.0, with 1.0 representing a perfect match and values <1.0 representing fractional abundances of identifed endmembers. Insert fig showing tree canopies and large pixels Spectral Angle Mapper For fine-scale spatial resolution imagery (1-2m), each individual pixel is likely to cover part of a single canopy. In this instance, the similarity between pixel spectra and those in the endmember library is used to assign a single species class to each pixel. Insert fig showing tree canopies and samll pixels